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KMID : 0351219920240040285
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Korean Journal of Infectious Diseases
1992 Volume.24 No. 4 p.285 ~ p.291
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A Study on Superoxide Anion Production by Peripheral Blood Neutrophil in Patients with Diabetes Mellitus
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신상원
유재명/강세용/우흥정/김우주/박승철
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Abstract
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It is generally believed that diabetics have increased susceptibility to infections, and have worse prognosis than nondiabetic population. But there is no evidence that immunologic competence is impaired in patients
with diabetes.
This study was designed to investigante the association of the superoxide anion production by peripheral blood neutrophild with the infections and other complications in diabetic patient. Superoxide anion production by peripheral neutrophils in
19
healthy subjects, and 40 diabetic patients(15 with infection and 25 without infection) were measured. The superoxide anion production, measured by spectrophotometric assay was expressed in absorbance at 550 nm.
The results were as follows:
1) After stimulation with 10E-6 FMLP (N-formyl methionyl leucyl phenylalanine), the superoxide production by neutrophil was higher in normal control group (17.14*3.54 nmole/30 min/1 10E6 neutrophils) than diabetic patient group (15.60*4.16
nmole/30
min/1 10E6 neutrophils).
2) Superoxide anion production was lower in the group of diabetic patients with infections (14.58*3.55 nmole/30 min/1 10E6 neutrophils) than the normal control group (17.14*3.54 nmole/30 min/1 10E6 neutrophils).
3) Superoxide anion production was lower in the group of diabetic patients with complication (14.13*3.33 nmole/30 min/1 10E6 neutrophils) than the group of diabetic patients without complication (17.05*4.45 mole/30 min/1 10E6 neutrophils).
In conclusion, the superoxide anion production defect in diabetic patient have associated with development of infections. And superoxide anion production may appear to have influence on the development of diabetic complication.
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KEYWORD
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